http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111138523-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f7e1236f72c6c121ab11b977ac2f0938 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-56 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-56 |
| filingDate | 2019-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf0eef81f3f29fd979712adcf20d6bd7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d8e914414ba621a328793c2ef29353b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_45b1d94010722e4937699a4863bfdce0 |
| publicationDate | 2020-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111138523-A |
| titleOfInvention | A method for purifying and preparing recombinant chicken interferon alpha from recombinant chicken interferon alpha renaturation solution |
| abstract | The invention discloses a method for purifying and preparing recombinant chicken interferon alpha from recombinant chicken interferon alpha renaturation solution, belonging to the field of biotechnology. , and nickel affinity chromatography. It is characterized in that the pretreatment of the recombinant chicken interferon alpha renaturation solution: adjusting the pH of the original solution to 3.0-3.5. The dialysate volume/renaturation solution volume=40, the dialysis time is 12-18h, and the dialysis is carried out at 4°C. The elution rate of cation exchange chromatography is 80-100 μg/mL. Nickel column affinity chromatography: Before loading, adjust the pH of the cation-exchange chromatography eluent to pH 7 with 1M NaOH. The PBS ratio of the equilibrium solution is: 20mM NaH2PO4, 0.5M NaCl, pH 7.4. The eluent was pH 7.0 with a concentration of 500 mM imidazole. The elution rate is 50-100 μg/mL. The purity of the recombinant chicken interferon alpha prepared by the method of the invention is more than 99.0%, the endotoxin is less than 100 EU/mL, and the biological activity is maintained at more than 99% before purification. While significantly improving the purity of interferon and significantly removing endotoxin, it maintains its biological activity well. |
| priorityDate | 2019-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.