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filingDate 2020-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3cc344bbdc9234ab4dc6e86c2cd9824
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publicationDate 2020-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111118093-A
titleOfInvention A kind of non-denatured yak skin collagen and preparation method thereof
abstract The invention discloses a method for preparing non-denatured yak skin collagen, which comprises the following steps: (1) pretreatment; (2) degreasing and impurity removal; (3) acidification and homogenization; (4) enzymatic hydrolysis; (5) Salting-out purification: add saturated brine to the supernatant obtained in step (4), and keep stirring until no white flocs continue to separate out, then let stand, centrifuge, and collect the precipitate; (6) isoelectric point purification: Dissolve the precipitate obtained in step (5) with acetic acid solution, then adjust pH to 6.5-7.0 with NaOH, stand, centrifuge, and collect the precipitate; (7) desalting; (8) drying. The method of the invention overcomes the disadvantage that the traditional preparation process of collagen causes damage to the triple helix structure of collagen, adopts the whole process of low-temperature preparation, uses acetic acid, enzymatic hydrolysis, freeze drying and other processes to obtain non-denatured yak skin collagen, and the technological process is simple and easy to operate. , industrial-scale production can be realized, and the prepared non-denatured yak skin collagen has good development and utilization prospects in the fields of biological dressings, drug delivery carriers and other medical biological fields.
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