http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111117985-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14143 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-864 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
| filingDate | 2020-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111117985-B |
| titleOfInvention | A method for splitting Cas9 and its application |
| abstract | The present invention discloses a method for splitting Cas9 and its application; the present invention provides a method for splitting the amino acid sequence of different segments of Cas9 protein at multiple sites. The Cas9 protein is spCas9 (D10A) protein, and the split The separated Cas9 protein is more convenient to be transported into the target cell/carrier, and can be recombined by means of intein or the like; accordingly, the present invention provides the proteome, fusion protein set and the method for expressing all the proteins obtained according to the method. The nucleic acid construct group of the fusion protein group and its vector group and engineered cell; the fusion protein group and the carrier can be used for the application in editing cell genes, or targeting localization or gene expression transcriptional activation or gene expression transcriptional inhibition in the preparation of pharmaceutical preparations for gene editing. |
| priorityDate | 2020-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 881.