http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111117974-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-16743 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43595 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-869 |
| filingDate | 2019-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111117974-B |
| titleOfInvention | Visual green fluorescent porcine pseudorabies virus and construction method thereof |
| abstract | The invention discloses a visual green fluorescent porcine pseudorabies virus and a construction method thereof, wherein the visual green fluorescent porcine pseudorabies virus comprises the following components in parts by weight: fluorescent protein is inserted between Met-1/Gly-2 of the minor backbone protein VP24 of pseudorabies virus. According to the invention, a CRISPR/Cas9 technology is utilized to introduce a fluorescent protein EGFP into a PRV genome, so that the generated recombinant virus has a green fluorescent label, and the positioning and movement of a single virus particle can be observed under fluorescence without an antibody or a fluorescein label, so that the track of the virus particle can be tracked by using green fluorescence. The recombinant PRV virus creates conditions for researching the detailed infection process of the PRV virus invading host. |
| priorityDate | 2019-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 189.