http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111103380-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_917117a8a15f1fb1836650fe7670cfbb |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-8822 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-045 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-8637 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-7266 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 |
| filingDate | 2019-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a06328609042174e60e19092dbff4a8b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d27369607f95211019f69171c5157fba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d2fe244568a7259984ccd3669982983 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a08b6bbed62a8f6045d594d557c4a91 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_657907019cc03a3d843489e473f72835 |
| publicationDate | 2020-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111103380-A |
| titleOfInvention | A method for determination of endogenous estradiol concentration in human plasma by liquid chromatography-mass spectrometry |
| abstract | The object of the present invention is to provide a method for accurately measuring the concentration of endogenous estradiol (Estradiol) in human plasma. The method of the invention breaks through the difficulty of endogenous interference detection, and has the characteristics of high efficiency, strong specificity, high accuracy and sensitivity. The invention adopts liquid mass spectrometry to measure the concentration of endogenous estradiol in human plasma, and the method includes: (1) selection of a substitute matrix; (2) determination of the concentration of endogenous estradiol in a control matrix (3) ) Preparation and pretreatment of working curve samples; (4) Preparation and pretreatment of quality control samples; (5) Separation and analysis conditions; (6) Using internal standard method, the concentration of a series of estradiol in the surrogate matrix is used as the horizontal Coordinates, the peak area ratio of estradiol to internal standard 17β-Estradiol-d4 is the ordinate, and the linear relationship between the theoretical concentration and the response is obtained by linear regression using the weighting factor 1/x2 for quantification. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112362770-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112362770-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113189242-A |
| priorityDate | 2019-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 53.