http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111103272-A

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filingDate 2019-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83df3ff9dac13f2296b2881ceb60fd97
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publicationDate 2020-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111103272-A
titleOfInvention System and method for real-time screening and measurement of cell-specific photosensitizing effects
abstract The present invention provides a real-time screening and measurement system and method for cell-specific photosensitivity effects. The method comprises using light A to be measured to stimulate cells to be tested that are fluorescently stained, and using light B and/or light C to perform fluorescence excitation on the cells, The excited fluorescence D is further imaged by CCD fluorescence, and transmitted to the computer for data processing and drawing, analyzing the real-time changes of the cell ion concentration to determine the existence of the photosensitive effect or its response; wherein, the cells to be tested are isolated cells, and the environment in which the cells are located There is an ion to be measured in the ion and an ion fluorescent indicator corresponding to the ion to be measured is added; the light A to be measured is a multi-parameter adjustable monochromatic light source; the wavelengths of the light A, B, C and D are spaced apart from each other. The method is fast and reproducible, and is a universal method for measuring photosensitive effects in cells.
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priorityDate 2018-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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