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filingDate 2019-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c01d9a09552e025f5b5d76a6d6fe120
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publicationDate 2020-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111100953-A
titleOfInvention LAMP detection primer set, kit and detection method of channel catfish herpes virus
abstract The invention discloses a LAMP primer set, a kit and a detection method for rapid detection of channel catfish herpes virus, which belong to the field of aquaculture disease prevention and control. The kit is composed of LAMP primer set, DNA polymerase, LAMP reaction solution, positive control, negative control and color developing agent. The LAMP primer set includes a pair of outer primers, a pair of inner primers and a pair of loop primers. The application steps of the detection kit in the detection of channel-tailed herpes virus include: preparation of channel-tailed herpesvirus DNA, isothermal gene amplification reaction, and result judgment by real-time fluorescence quantitative PCR instrument. The kit of the invention has the advantages of simple operation, rapid detection, good specificity, high sensitivity and reliable results, can play an important role in the early rapid diagnosis and real-time monitoring of channel catfish herpes virus disease, and is suitable for channel catfish breeding sites to quickly detect pathogens Detection use.
priorityDate 2019-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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