http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111100876-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_50c84f5a031aef3f5b721b0272c3bd94 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-15043 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2810-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1137 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y307-01002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-14 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
| filingDate | 2018-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_74a8a7fa0193604addf643675ff81263 |
| publicationDate | 2020-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111100876-A |
| titleOfInvention | CRISPR-Cas9 specific knockout method of FAH gene and specific sgRNA |
| abstract | The present invention relates to a method for CRISPR-Cas9 to specifically knock out FAH gene and specific sgRNA. The method is applied to specifically knock out FAH gene in pigs and rabbits. The specific steps include: step S1: selection of sgRNA target sequence of FAH gene and Design; Step S2: construct a CRISPR-Cas9 vector of FAH gene; Step S3: obtain a pseudotyped lentivirus expressing FAH sgRNA and Cas9 protein; Step S4: infect target cells and detect the effect of FAH gene knockout. The target sequences of the specific sgRNA on the pig and rabbit FAH genes conform to the sequence arrangement of 5'-N(20)NGG-3'. The invention uses the specific sgRNA in the method for CRISPR-Cas9 to specifically knock out the FAH gene of pigs and rabbits, which can quickly, accurately, efficiently and specifically knock out the FAH genes of pigs and rabbits respectively, and effectively solve the problem of constructing FAH gene knockout. In addition to the technical problems of pigs and rabbits with long cycle and high cost. |
| priorityDate | 2018-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 63.