http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111088319-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-24 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-24 |
| filingDate | 2020-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111088319-B |
| titleOfInvention | Inactivated virus sample RNA preservation solution and preparation method thereof |
| abstract | The invention discloses an inactivated virus sample RNA preservation solution and a preparation method thereof, wherein the preservation solution comprises the following components: the sodium citrate, the proteinase K, the concentrated sulfuric acid, the ethylene diamine tetraacetic acid and the ammonium sulfate, wherein the final concentration of the sodium citrate is 10 mM-35 mM, the final concentration of the proteinase K is 50-800 mu g/ml, the final concentration of the concentrated sulfuric acid is 0.5% -5% (V/V), the final concentration of the ethylene diamine tetraacetic acid is 5 mM-50 mM, and the final concentration of the ammonium sulfate is 45% -80% (W/V). The preserving fluid is safe and nontoxic, simple in preparation and convenient to use; the virus can be inactivated after entering the preservation solution without refrigeration, transportation and inactivation treatment; RNA degradation can be avoided at room temperature, and repeated freezing and thawing can be performed without affecting the RNA extraction quality; the RNA degradation can be prevented in the extraction process, the integrity of the extracted RNA is better, and the method is particularly suitable for collecting the epidemic virus sample with strong infectivity. |
| priorityDate | 2020-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.