http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111088252-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2019-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111088252-B |
| titleOfInvention | Specific labeling method of 5-hydroxymethyl uracil on DNA |
| abstract | The invention discloses a specific labeling method of 5-hydroxymethyl uracil on DNA, belonging to the technical field of molecular biology. The specific labeling method utilizes 5-hmU kinase separated from pseudomonas aeruginosa bacteriophage M6 to realize the transfer of gamma-thiophosphate from 5-O-thiophosphoric acid adenosine triphosphate to 5-hmU and generate 5-thiophosphoric acid methyl uracil; then, the generated 5-thiophosphoric acid methyl uracil is characterized by a liquid chromatography-tandem mass spectrometry method, and the 5-hydroxymethyl uracil on the DNA is specifically marked by utilizing a cross-linking chemical labeling sulfhydryl. The method of the invention effectively overcomes the defects of large demand of the detection sample, harsh chemical reaction conditions, low reaction efficiency and the like. |
| priorityDate | 2019-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.