http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111088234-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2020-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111088234-B |
| titleOfInvention | A kind of double-stranded DNA peptide ligase dDPlaseII and using method |
| abstract | The present invention provides a novel double-stranded DNA peptide ligase dDPlaseII, which can be used to catalyze the covalent connection of a specific double-stranded DNA and a specific polypeptide, and its enzymatic properties and use methods. The amino acid sequence of the dDPlaseII enzyme is shown in SEQ ID NO: 8, and the corresponding gene coding sequence is shown in SEQ ID NO: 7; A specific DNA double-strand whose 5'-terminal deoxyribonucleotide C is in a phosphorylated state and a specific polypeptide chain whose C-terminus starts with 7 peptides of N'‑MANCEHL‑C' and catalyzes the covalent bond between the two Together; double-stranded DNA and polypeptide ligation products have characteristic absorption peaks at 372nm wavelength, which can be used for the determination of dDPlaseII ligase activity. The dDPlaseII ligase buffer is composed of 450mM Tris‑HCl, 100mM Mg 2+ , 80mM NaCl, 20mM ATP and 8mM Triton X‑100 at pH 7.8. The optimum reaction temperature range is 35℃‑45℃. The ligation reaction The time is 3min‑10min. The novel double-stranded DNA polypeptide ligase provided by the invention can be used as a tool enzyme for genetic engineering and gene analysis. |
| priorityDate | 2020-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 553.