http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111087468-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-505 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-56 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56983 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13 |
| filingDate | 2020-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111087468-B |
| titleOfInvention | PRRSV broad-spectrum neutralizing monoclonal antibody 5D9 and application thereof |
| abstract | The invention adopts PRRSV virus liquid-SD 16 as immunogen to immunize Balb/c mice. After cell fusion and virus infection, Marc145 cells are screened and cloned to obtain a positive hybridoma cell line which efficiently secretes the monoclonal antibody, and a mouse monoclonal antibody 5D9 is obtained. The subtype of the monoclonal antibody 5D9 was determined to be an IgM type monoclonal antibody by ELISA technique. The PRRSV-I type and PRRSV-II type viruses infect Marc145 cells, and the monoclonal antibody is used for detection by using an IFA technology, so that the monoclonal antibody 5D9 is proved to have broad-spectrum reactivity to the PRRSV-I and PRRSV-II type viruses. Then, the monoclonal antibody is used for virus neutralization experiments, and Western blot technology and qPCR technology are utilized to prove that the monoclonal antibody has neutralization activity on PRRSV-I and PRRSV-II viruses, so that virus invasion can be prevented, and an organism is protected from infection. |
| priorityDate | 2020-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 599.