http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111077251-B

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-89
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-89
filingDate 2019-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2023-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2023-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111077251-B
titleOfInvention A target protein-based mesoporous biofilm column and its application in screening active components in natural products
abstract The invention discloses a target protein-based mesoporous biofilm chromatographic column and its application in screening active components in natural products. The preparation method of the mesoporous biofilm chromatographic column based on the target protein is: the mesoporous silicon oxide material is mixed with α-glucosidase solution, glutathione solution, and sodium acetate buffer solution, and the reaction is stirred under ice bath , to obtain a mesoporous silica material loaded with α-glucosidase receptors; fully dissolving soybean lecithin and cholesterol in chloroform to obtain a mixed solution; impregnating the obtained mesoporous silica material loaded with α-glucosidase receptors in In the obtained mixed solution, the organic solvent is evaporated under reduced pressure, and a layer of phospholipid film is formed on the surface of the mesoporous silicon oxide material loaded with α-glucosidase receptors, and then washed and dried to obtain a mesoporous stationary phase covered with a phospholipid film. Pack the column to obtain a mesoporous biofilm chromatographic column based on the target protein. The target protein-based receptor biofilm chromatographic column of the present invention is applied to screening active components of natural products.
priorityDate 2019-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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