http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111073968-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_71de0b426be6a23c375c5536cbe2ad60 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-106 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6883 |
| filingDate | 2018-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d85bf79e375a769a45714e74ea254fff http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_809dfb7b687b1777e543ca7045e01784 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1566eb41c769d26bee960ccf79042d7 |
| publicationDate | 2020-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111073968-A |
| titleOfInvention | Method for distinguishing individual administration of benazepril by using primer composition to perform mass spectrometry |
| abstract | The invention provides a method for simultaneously detecting a plurality of sites by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) by utilizing extension primers with different molecular weights at different Single Nucleotide Polymorphism (SNP) sites, and finally obtaining a method for distinguishing benazepril individualized medication by using a primer composition through mass spectrometry, which comprises the following steps: respectively designing a multiple amplification primer and an extension primer according to a plurality of target SNP sites to be detected; preparing a multiple amplification primer reaction system and an extension primer reaction system; in a reaction system, a plurality of sets of primers are used for simultaneously and respectively carrying out amplification and single base extension reaction on a plurality of target SNP sites; and (3) carrying out flight time mass spectrometry on the product obtained after the single base extension reaction, identifying the genotype of SNP (single nucleotide polymorphism) related to different drug metabolism according to the products of the extension primers with different molecular weights represented by mass spectrum peaks, and guiding the individual administration of the antihypertensive drug benazepril. The method can simultaneously detect 9 SNP sites related to benazepril drug metabolism, and has the advantages of low cost, no need of synthesizing probes, short time consumption, simple and convenient result analysis and extremely wide application field. |
| priorityDate | 2018-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 117.