http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111073822-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_be2c55a9de09e08e3404c0a5a1ae4dc7 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y102-0101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0008 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 2019-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_12cb21dfa11a59e0c99623c294f06950 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c4fd574102eb16755f1ec3e95bf35eb2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee489ddc574e972d34292f86508789a2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee606e23a9de0f3712082d7a770e0912 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ceb5fbdff0c6f6e4e06001ddb2a27dae |
| publicationDate | 2020-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111073822-A |
| titleOfInvention | A kind of Saccharomyces cerevisiae engineering bacteria for producing dihydroartemisinic acid and its construction method and application |
| abstract | The invention relates to the technical field of microorganisms and discloses an engineering bacterium of Saccharomyces cerevisiae for producing dihydroartemisinic acid. Based on Saccharomyces cerevisiae transformed by the heterologous synthetic pathway of dihydroartemisinic acid, the engineering bacteria of the present invention makes the original ALDH1 expression sequence to express the mutated ALDH1 H194R or ALDH1 V247F sequence. The invention performs site-directed mutation on the key gene ALDH1 of the pathway to obtain a gene with better selectivity, makes the metabolic pathway more preferred for the synthesis of dihydroartemisinic acid, and reduces the proportion of artemisinic acid. Two mutants, ALDH1 H194R and ALDH1 V247F , were demonstrated in the present invention to increase the ratio of dihydroartemisinic acid/artemisinic acid. On this basis, the fusion expression of DBR2 and ADH1, and the fusion expression of mutant ALDH1 and DBR2 can further improve the ratio of dihydroartemisinic acid/artemisinic acid without sacrificing the yield of dihydroartemisinic acid. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113462724-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112852860-A |
| priorityDate | 2019-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 145.