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publicationDate 2020-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111066775-A
titleOfInvention A method for cryopreserving animal sperm
abstract The present invention relates to a method for refrigerating and preserving animal sperm, comprising the following steps: (1) putting the collected animal semen into a thermos bottle, and keeping the temperature at 20-35°C; (2) storing the semen in step (1) Measure the density with a spectrophotometer, dilute it with animal sperm diluent according to the sperm count of 300-10 million/mL, and fill the diluted semen into a frozen fine tube with a filling and sealing machine and seal it; (3) Step (2) Put the semen in the semen into the cooling cabinet, cool down at a rate of 0.1-0.5°C/min, and lower it to 0-4°C for use. The invention solves the problems of unclear observation field, easy contamination, short survival time and irregular use of antibiotics, resulting in the generation of super bacteria when the animal sperm is preserved by the traditional method.
priorityDate 2016-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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