http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111057745-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 |
| filingDate | 2019-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111057745-B |
| titleOfInvention | A high-throughput screening method for the hemotoxic effect of a substance |
| abstract | A high-throughput screening method for the blood toxicity effect of a substance, comprising: diluting plasma with a reaction buffer and mixing it with a substance to be tested, setting a solvent control group at the same time, and incubating at 35-40° C. for 0-60 min; adding plasma stimulation After peptidylase detects the substrate, monitor the fluorescence change of the enzyme reaction within 5-30min; draw a curve with the test time as the abscissa and the fluorescence value as the ordinate, and obtain the enzyme reaction rate through linear fitting. The reaction rate is compared to the enzyme reaction rate of the solvent control to determine whether the test substance exhibits an activating effect on plasma prokallikrein. The method has the advantages of short time-consuming, simple operation, high sensitivity, good reproducibility of results, wide quantitative response range, suitable for high-throughput detection, etc. It can be used in the field of environmental health risk assessment of chemicals or pollutants. |
| priorityDate | 2019-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.