http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111057700-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-20 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-12 |
| filingDate | 2019-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111057700-B |
| titleOfInvention | Method for immobilizing lipase interface based on natural polysaccharide particles |
| abstract | The invention discloses a method for immobilizing a lipase interface based on natural polysaccharide particles. Dissolving chitosan in acetic acid solution, shearing and homogenizing to disperse chitosan particles uniformly; adding genipin, crosslinking the chitosan particles, and crosslinking under the condition of keeping the temperature and the pH constant to form a dispersion liquid of the crosslinked chitosan particles; carrying out high-pressure micro-jet treatment on the dispersion liquid of the cross-linked chitosan particles to disperse the particles; adding mineral oil into the obtained chitosan particle dispersion liquid to form oil-in-water Pickering emulsion, adding lipase, centrifuging the emulsion for layering, taking an emulsion layer to obtain the core-shell structure microspheres with the surfaces coated with the lipase and the chitosan particles, and realizing the immobilization of a lipase interface. Compared with free enzyme, the microsphere can stabilize an oil-water interface to form emulsion in a two-phase system, the activity of catalyzing the reaction of lipid hydrolysis at the oil-water interface is greatly improved, the processing stability is high, an organic solvent is not needed for solubilizing a lipid substrate, and the microsphere can be directly applied to food processing. |
| priorityDate | 2019-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 71.