http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111057657-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2e9297c8f247b3724a04ec3c3130f182 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-44 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate | 2018-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_179b11d8bd7675704304b5d17a06406c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_456b629121bb871d15d0821fa384f5a1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68c112f2ff925554ca728a7f565a17ba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af666f9c3ad22c94b3bb2dbe7617637b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c072ad1522e68d0b90b9228ed22495d2 |
| publicationDate | 2020-04-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111057657-A |
| titleOfInvention | Method for optimizing carbon source of staphylococcus carnosus high-density culture medium and application of method |
| abstract | The invention discloses a method for optimizing a carbon source of a staphylococcus carnosus high-density culture medium and application thereof. The carbon source that staphylococcus carnosus can utilize was comprehensively screened from 95 different carbon sources, colloids and tetrazoles contained in the identification plate by a full-automatic microbial identification system (Biolog) method. Based on the principle of color reaction, the instrument reads the absorbance at 590 nm. In the Biolog method, a single colony of bacteria to be detected is purified by using a BUG solid plate, then the single colony is picked by using a cotton swab and uniformly mixed in inoculation liquid containing gram-positive bacteria, the bacteria are diluted, then the diluted bacteria liquid is sucked by using a discharging gun and inoculated on a GP2 positive 96 pore plate, continuous culture is carried out for 15 hours, reading is carried out every 1 hour, then the discoloration condition of a culture hole is observed, a carbon source capable of being utilized by the bacteria is judged, then statistical analysis is carried out on specific numerical values, a high-quality carbon source capable of being utilized by staphylococcus carnosus is selected by using visual numerical values, and then high-density culture is carried out by using the carbon source. |
| priorityDate | 2018-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 219.