http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111044732-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6bb64ea2ef66bd74dd187467b63bfbd3 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-70589 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2469-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56972 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-1434 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N15-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2019-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa856e69866850c2728899211386052f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9862cf1201ef3287da83c680399ff349 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b05dd3a2d315e6cfec8281acf42295e0 |
| publicationDate | 2020-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111044732-A |
| titleOfInvention | Kit for CD45 antigen detection by flow cytometry and preparation method thereof |
| abstract | The invention discloses a kit for CD45 antigen detection by flow cytometry and a preparation method thereof, including antibody, fluorescein, fluorescent protein, preservative, stabilizer, cross-linking agent A, cross-linking agent B and buffer liquid. Dissolve fluorescein with DMSO, and incubate with fluorescent protein at a ratio of 0.5-10:0.8-3 at 4-40°C in the dark for 1-18 h to obtain a tandem dye; after removing unreacted fluorescein by dialysis The tandem dye and cross-linking agent A were incubated in the dark at 4-40°C for 1-18 h at a ratio of 1-10:20-100 to obtain an activated tandem dye; ‑8:4‑100 ratio, incubate in the dark at 4‑40°C for 1‑18h to obtain an activated antibody, and the activated tandem dye and antibody at a ratio of 0.3‑4:0.5‑5, at 4‑ Incubate in the dark for 1-18 h at 40°C to obtain a CD45 antigen detection kit. Whole blood is gated by fluorescently labeled antibodies to recognize CD45+ cells, facilitating subsequent use of other CD-series reagents for cell clustering. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112098640-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112098640-B |
| priorityDate | 2019-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 74.