| abstract |
The invention discloses a rapid constant temperature detection method, a primer set and a kit for Bacillus cereus. The method includes: extracting genomic DNA from a sample to be tested; using the genomic DNA as a template, using a primer set capable of amplifying a Bacillus cereus specific sequence as a primer, and performing a constant temperature amplification reaction under an enzyme reaction system; By judging whether the reaction result is positive, it is determined whether Bacillus cereus exists in the sample to be tested. The detection method of the invention has the advantages of high sensitivity and high specificity, short detection time, simple result determination, convenient operation, low cost and wide application prospect. |