| abstract |
The invention discloses a rapid constant temperature detection method, primer set and kit for Vibrio vulnificus. The method is as follows: extracting genomic DNA from a sample to be tested; using the genomic DNA as a template, using a primer set capable of amplifying a specific sequence of Vibrio vulnificus as primers, and performing a constant temperature amplification reaction under an enzyme reaction system; Determine whether the reaction result is positive, and determine whether Vibrio vulnificus exists in the sample to be tested. The detection method of the invention has the advantages of high sensitivity and high specificity, short detection time, simple result determination, convenient operation, low cost and wide application prospect. |