http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111040988-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6a8b75500e4424e0f8f3abd3c2d5d6c3 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-999 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-02 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-067 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2019-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b4da3bce2af3778fe6723ece58dfb8a |
| publicationDate | 2020-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111040988-A |
| titleOfInvention | Culture method for inducing differentiation of embryonic stem cells into liver cells |
| abstract | The invention discloses a culture method for inducing embryonic stem cells to differentiate into liver cells, which comprises the following steps: during the culture process, the baijiu grass saponin R is used, and the culture medium further comprises iron dextran injection. The induction culture steps are as follows: (1) selecting a mouse ESC line, and firstly culturing in a human embryonic stem cell conditioned medium for 3 d; (2) adding 20-30ng/mL baijiu grass saponin R and 20-30ng/mL iron dextran injection into the culture solution to induce for 5 days so as to differentiate towards the direction of hepatic cells; (3) the differentiated hepatocytes were continued to be cultured for 5d with 1 change of medium per day. |
| priorityDate | 2019-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.