http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111024935-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f68ddaa0ddd894016af217422d657c22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56966 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
| filingDate | 2019-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fcc257b0803807454c049d0666a2a51e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_404f708d243e420d53361ed766dc8904 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_917ac289b621992392e692e117c7ce41 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49d7fbc36849c4a2229b0ebcf77badd7 |
| publicationDate | 2020-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111024935-A |
| titleOfInvention | A zebrafish embryonic heart immunofluorescence labeling method |
| abstract | The invention discloses a zebrafish embryo heart immunofluorescence labeling method, which is characterized by comprising the following steps: S1. Take a clean glass slide and draw a circle with a diameter of 1 cm with a fluorescent marker, add paraformaldehyde in the circle, and place the Put the slides in a humid box; S2, fix the zebrafish heart; S3, wash; S4, block; S5, repeat step S3; S6, add primary antibody, incubate overnight at 4°C; S7, place in a humid box at room temperature the next day; S8 , repeat step S3; S9, add secondary antibody, and incubate in the dark at room temperature; S10, repeat step S3; S11, image under a fluorescence microscope or laser confocal, and take a picture to record the result. The invention solves the problems that the heart is not stained when the whole fish is subjected to immunofluorescence, the heart is easily lost during the PBST washing process, the sample cannot be stored for a long time after the secondary antibody is incubated, and the amount of antibody used is large. The samples can be stored for two weeks after mounting, and the individual hearts can be directly observed under the microscope to ensure the accuracy of the experiment. |
| priorityDate | 2019-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.