http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111024868-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-88 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 |
| filingDate | 2020-01-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111024868-B |
| titleOfInvention | Method for detecting content of alpha-high nojirimycin in white tree medicinal material |
| abstract | The invention provides a method for detecting the content of alpha-high nojirimycin in a white tree medicinal material. The method comprises the following steps: preparing a reference solution: precisely weighing an alpha-high nojirimycin reference substance, adding a solvent for dissolving, and preparing a reference substance solution with required concentration; preparing a test solution: taking the medicinal material powder of the white tree to be detected, adding an extraction solvent for extraction, centrifuging, taking supernatant, and fixing the volume to obtain a test solution; content determination: measuring peak areas of alpha-high nojirimycin in a reference solution and a test solution by liquid chromatography-tandem mass spectrometry, and calculating the content of the alpha-high nojirimycin in the white tree medicinal material according to an external standard method. The method adopts the liquid chromatography-tandem mass spectrometry to realize the resolution of the polyhydroxy alkaloid substance alpha-high nojirimycin and 4 chiral isomers thereof for the first time, realizes the direct qualitative and quantitative analysis of the alpha-high nojirimycin, and has good system applicability, stability, repeatability and reproducibility. |
| priorityDate | 2020-01-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.