| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3c96af599b7d29b6715a68a0b4c224a1 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-54 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-52 |
| filingDate |
2018-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e9283e5ae69a6989429f7cbd29d0a5e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7791c441643825ea6f5d2c102c402157
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d2baa88df51bb61a20a1fc1c062669b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df02935461531753d8fe23ec669dd463
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6282f24379817521bf4d50cdb8799bdd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28ba3759e624d9f60f21d6e0ed2fb3e4 |
| publicationDate |
2020-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-111019965-A |
| titleOfInvention |
Genetically modified engineering bacteria of neomycin biosynthetic gene cluster and its application |
| abstract |
The invention provides a method for constructing a high-yielding neomycin-producing engineering strain Streptomyces fradiae, the method comprising introducing a neomycin biosynthesis gene cluster of Streptomyces fradiae into wild-type Streptomyces fradiae. The method may also include genetically engineering the neomycin biosynthesis gene cluster of Streptomyces flexneri to be introduced into wild-type Streptomyces flexneri, the genetic engineering may include genetically engineering the neomycin biosynthesis gene cluster in the neomycin biosynthesis gene cluster. The negative regulatory gene neoI of the neomycin block or the negative regulatory gene neoI in the neomycin biosynthesis gene cluster is blocked, and the strong promoter is used to drive the transcription of the key enzyme-encoding gene neoE‑neoD co-transcription unit. The present invention also provides the neomycin-producing Streptomyces flexneri obtained by the above method. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111690582-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111690582-A |
| priorityDate |
2018-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |