http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111004318-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-635 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-635 |
filingDate | 2019-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111004318-B |
titleOfInvention | Purification method of rhPTH (1-34) protein stock solution |
abstract | The invention discloses a purification method of rhPTH (1-34) protein stock solution. The method comprises the steps of thallus crushing, nickel ion chelating affinity chromatography, fusion protein enzyme digestion, anion exchange chromatography, reversed phase chromatography and cation exchange chromatography, wherein the thallus crushing specifically comprises the following steps: and (2) taking the thalli, resuspending the thalli by using a buffer solution A, breaking the thalli by using a high-pressure homogenizer, centrifuging the broken thalli, collecting supernate, filtering the supernate by using a 0.45-micrometer filter element, and collecting filtrate, wherein the buffer solution A comprises phosphate buffer solution, NaCl, imidazole and water, the concentration of the phosphate buffer solution is 10mmol/L, NaCl and is 500mmol/L, the concentration of the imidazole is 50-60 mmol/L, and the pH value of the buffer solution A is 8.0. By applying the technical scheme of the invention, the total yield of the rhPTH (1-34) protein stock solution is improved to more than 50 percent, and the purity is improved to more than 99.5 percent. |
priorityDate | 2019-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 803.