http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111004246-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K2211-1033 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K11-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D491-107 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D491-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09K11-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2019-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111004246-B |
| titleOfInvention | Rhodamine pH fluorescent probe for monitoring mitochondrial autophagy, preparation and application thereof |
| abstract | The invention relates to the technical field of pH fluorescent probes, in particular to a rhodamine pH fluorescent probe for monitoring mitochondrial autophagy, and preparation and application thereof. In order to solve the problems that a plurality of fluorescence probes for detecting the pH change of the lysosome are not suitable for monitoring cells for a long time, few lysosome targeting pH fluorescence probes which can be actually used for monitoring mitophagy are few, and the like, the invention provides a preparation method which comprises the following steps: dissolving N-ethyl p-toluenesulfonate derivative of rhodamine B and N-aminomorpholine in dichloromethane, heating and refluxing, cooling to room temperature, decompressing, carrying out rotary evaporation to remove the solvent to obtain a crude product, and purifying the crude product by silica gel column chromatography to obtain a light yellow solid as a pH fluorescent probe. The fluorescent probe has good cell membrane permeability, can target and mark lysosome, realizes visual monitoring of the autophagy process of the mitochondria by detecting the change of the pH of the lysosome, and has potential application prospect in the study of the pH physiology and pathology of the lysosome. |
| priorityDate | 2019-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.