| abstract |
In one aspect, described herein is an antigen presenting cell (APC) comprising exogenous nucleic acid encoding one or more candidate antigens, wherein the one or more candidate antigens are expressed with MHC class I or MHC class II molecules and presentation; a molecular reporter of granzyme B (GzB) activity; and c) caspase-activated deoxyribonuclease (CAD)-mediated DNA degradation, CAD knockout, or caspase knockout (e.g., caspase 3 knockout) exogenous inhibitor. In another aspect, described herein is a system for detecting the presentation of recognized antigens by antigen presenting cells to cytotoxic lymphocytes or NK cells. |