http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110974948-A

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filingDate 2019-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d04021fd876d1d1e911e1fd5e7e1eb75
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publicationDate 2020-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110974948-A
titleOfInvention A kind of aquatic Lahenia inactivated vaccine and application
abstract The invention relates to an inactivated vaccine of aquatic Lahenia, and its preparation steps are as follows: taking aquatic Lahenia, culturing it in LB liquid culture medium, and inoculating 2% of the culture liquid into fresh LB liquid culture medium for 28 Incubate at ‑30℃ for 16‑36h, add formaldehyde, inactivate at 28‑30℃, 140r/min shaker; centrifuge the inactivated bacterial liquid, discard the supernatant, add normal saline and mix well, centrifuge, and proceed Washing is repeated 2-4 times with normal saline to wash the formaldehyde, and the formaldehyde-inactivated vaccine of aquatic Lahne bacterium is obtained. The aquatic Lahenia inactivated vaccine of the invention has no infectivity, cannot proliferate in the body, and has high safety. This vaccine can kill other possible contaminating biological factors and is not infectious. The vaccine has a good immune protection effect, is helpful for the prevention and treatment of aquatic lanemycosis in aquatic animals, and has more practical value.
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