http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110964760-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2e9297c8f247b3724a04ec3c3130f182 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-01 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-225 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
filingDate | 2018-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_179b11d8bd7675704304b5d17a06406c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68c112f2ff925554ca728a7f565a17ba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_058ede696de67ad34266b1b0ed9b1aff http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af666f9c3ad22c94b3bb2dbe7617637b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a528d7e05e02acbf05ebe7f4767ba820 |
publicationDate | 2020-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110964760-A |
titleOfInvention | Mutagenesis breeding method of high-yield gamma-aminobutyric acid strain |
abstract | The invention discloses a method for breeding Lactobacillus brevis with high yield of gamma-aminobutyric acid (GABA) by secondary mutagenesis, which comprises normal-pressure room-temperature plasma mutagenesis (ARTP) of Lactobacillus brevis and a screening method of high-yield strains. The invention is characterized in that on the basis of high-yield strains obtained by mutagenesis of a Multifunctional Plasma Mutagenesis System (MPMS) (nitrogen as a carrier) in the previous period, higher yield of gamma-aminobutyric acid is obtained by mutagenesis of ARTP (helium as a carrier) again, and a 5L fermentation tank amplification experiment is carried out on the high-yield strains, so that the yield of target products of the strains can be higher by the mutagenesis method of secondary ARTP. Compared with the conventional mutagenesis method, the mutagenesis method of the strain has high efficiency, the screening process is simple and quick, the yield of the obtained mutagenesis strain gamma-aminobutyric acid is higher, the final yield of the obtained mutant strain is 115.72g/L, 9.80 percent of the mutant strain is improved than that of the original strain, the molar conversion rate is 93.95 percent, 3.93 percent of the mutant strain is improved than that of the original strain, and a solid theoretical basis and data reference are provided for the improvement of industrialization. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111778191-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112826076-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111560367-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111778191-B |
priorityDate | 2018-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 63.