http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110964702-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-14043 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0004 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y121-03003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y121-03008 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-866 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-26 |
| filingDate | 2019-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110964702-B |
| titleOfInvention | Application of Diels-Alder reaction enzyme and preparation method and application of mutant thereof |
| abstract | The invention provides application of Diels-Alder reaction enzyme and a preparation method and application of a mutant thereof, belonging to the technical field of genetic engineering. The invention discovers that Diels-Alder reaction enzyme with an amino acid sequence shown as SEQ ID No.1 can catalyze a substrate to synthesize a natural D-A product and can catalyze and synthesize an unnatural D-A product, and further discovers that a substrate diene of the D-A reaction enzyme also comprises biphenyls, flavonoids and benzofuran. The present invention extends the scope of application of substrates for D-A reaction enzymes and provides mutants of the enzyme which have been found to be capable of catalyzing the synthesis of natural or unnatural D-A products from the substrates. The D-A reaction enzyme and the mutant thereof provided by the invention are beneficial to developing and utilizing the value of a D-A product, and provide possibility for synthesis of other important chemical precursors or natural products containing six-membered rings. |
| priorityDate | 2019-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 456.