http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110964090-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-26 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-15 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-34 |
| filingDate | 2019-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110964090-B |
| titleOfInvention | A protein initiation factor IF3 mutant that promotes N-acetylglucosamine production and its application |
| abstract | The invention discloses a protein initiation factor IF3 mutant for promoting the production of N-acetylglucosamine and its application. The mutant of the present invention is that on the parent whose amino acid sequence is shown in SEQ ID NO.2, the asparagine at position 158 is mutated into glutamic acid, the alanine at position 161 is mutated into arginine, and the 162-position alanine is mutated into arginine. Aspartic acid was mutated to glycine, and phenylalanine at position 163 was mutated to serine. The invention provides a protein initiation factor IF3 mutant that promotes the production of N-acetylglucosamine, and a genetically engineered strain capable of improving the production of N-acetylglucosamine. By overexpressing the protein initiation factor IF3 involved in carbon metabolism regulation, The extracellular accumulation of N-acetylglucosamine is increased, and its concentration can reach up to 28.3g/L, which lays a foundation for further metabolic engineering of Corynebacterium glutamicum to produce glucosamine. The construction method of the recombinant Corynebacterium glutamicum provided by the invention is simple, easy to use, and has a good application prospect. |
| priorityDate | 2019-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 195.