| abstract |
The present disclosure relates to methods of altering the isoelectric point of an antibody. The method comprises providing an antibody comprising a first polypeptide comprising a heavy chain variable region and a second polypeptide comprising a heavy chain variable region; and, according to the Kabat numbering system, in the first polypeptide of the antibody In at least one of the polypeptide and the second polypeptide, one of positions 7, 9, 11, 14, 41, 70, 74, 82a, 84 and 113 of the heavy chain variable region (VH) is substituted or a plurality of amino acid residues, wherein the substitution increases or decreases the isoelectric point of the antibody. |