http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110951852-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2019-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110951852-B |
| titleOfInvention | Single-base serial extension flow targeted sequencing |
| abstract | The invention discloses a single-base continuous extension flow targeted sequencing method. The method utilizes single-stranded nucleotide fragments containing complementary sequences of nucleic acid fragments to be tested, primers, microspheres, nucleic acid polymerase, fluorescently labeled ddNTP, Ribose 3'-OH protected fluorescently labeled dNTP, ribose 3'-OH protected dNTP, ribose 3'-OH deprotection agent, etc., through the single base extension of the primer, a series of suitable for flow cytometry The sequencing microspheres used to detect the nucleic acid base sequence, and these sequencing microspheres are further detected by the flow cytometer, and the base sequence of the nucleic acid fragment to be tested can be obtained. Compared with existing sequencing methods, the present invention has significant advantages such as simplicity, accuracy, and easy interpretation of data, and can be widely used in gene detection, microbial inspection, genetics, exons, single nucleotide polymorphism (SNP), genome Nucleic acid sequencing fields such as biology and proteomics. |
| priorityDate | 2019-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 139.