http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110951803-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01158 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2468 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01081 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2402 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 |
| filingDate | 2019-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110951803-B |
| titleOfInvention | Method for preparing neoagarobiose by combined utilization of agarase, recombinant host cell and application of recombinant host cell and expression vector |
| abstract | The invention discloses a method for obtaining high-purity neoagarobiose by degrading agar or crude agar (red algae polysaccharide extract) by combining endo-type agarase and exo-type agarase. The invention relates to agarase AgaA and bifunctional enzyme AgaB, wherein the AgaA is an incision agarase, can rapidly degrade agar or agarose to generate neoagarobiose, tetrasaccharide and hexasaccharide, and the amino acid sequence of the agarase AgaA is shown in SEQ ID No. 1; AgaB is bifunctional excision agarase, has the activity of degrading agarose and porphyra polysaccharide (namely sulfated agarose), can degrade agar or crude agaragar (red algae polysaccharide extract) or oligosaccharide thereof to generate a single product neoagarobiose, and has an amino acid sequence shown in SEQ ID No. 2. Meanwhile, the invention also discloses a method for recombining, expressing and preparing the agarase by utilizing the genetic engineering technology. The method for preparing high-purity neoagarobiose and the specific agarase (or the laver polysaccharidase) have wide application prospects in the field of preparing agar oligosaccharides. |
| priorityDate | 2019-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 534.