http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110951743-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y202-01006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1022 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8218 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-46 |
| filingDate | 2019-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110951743-B |
| titleOfInvention | Method for improving plant gene replacement efficiency |
| abstract | The invention discloses a method for improving the gene replacement efficiency of a plant. The method comprises the following steps: introducing the esgRNA, Cas9 nickase, a screening agent resistance protein and donor DNA into a target plant; an esgRNA targeting DNA fragment A target sequence; cas9 nickase is co-expressed with a screener resistance protein; the donor DNA sequentially comprises a DNA fragment A target sequence, a DNA fragment B and a DNA fragment A target sequence; the DNA fragment B is a DNA molecule obtained by mutating the DNA fragment A by one or more bases; under the guidance of esgRNA, Cas9 nickase generates single-stranded DNA nicks at a DNA fragment A target sequence in a target plant genome and a DNA fragment A target sequence in donor DNA, and replaces the DNA fragment A in the target plant genome with the DNA fragment B through a repair mechanism in a target plant body, so that plant gene replacement is realized. |
| priorityDate | 2019-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1032.