http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110951683-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e1d50f073aacf43a633689ec3462ada4 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0664 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 |
| filingDate | 2020-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26a475253e561e498f35a3d563c6483d |
| publicationDate | 2020-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110951683-A |
| titleOfInvention | A kind of preparation method of dental pulp stem cells |
| abstract | The invention belongs to the technical field of cell culture, and in particular relates to a method for preparing dental pulp stem cells. The method comprises: taking out dental pulp tissue from a tooth and cutting the dental pulp tissue into pieces; placing the cut dental pulp tissue in the dental pulp In the stem cell culture medium, centrifuge, and then remove the supernatant to obtain dental pulp tissue fluid; add collagenase type I to the dental pulp tissue fluid, and digest at a specific temperature; continue to add dental pulp stem cells to the digested dental pulp tissue fluid for culture base, and purified to obtain a dental pulp stem cell single cell suspension; dental pulp stem cells were prepared based on the dental pulp stem cell single cell suspension. The invention uses the enzymatic digestion method to prepare the dental pulp stem cells, and the enzymatic digestion method has the advantage of higher efficiency than the tissue adherent method for culturing cells, and the cells can adhere to the wall in a short time, so that the subsequent stem cell culture is simpler and faster; The cell immunogenicity is low, suitable for a wide range of people, and the subsequent expansion is convenient, and the high cell activity meets the requirements of clinical application. |
| priorityDate | 2020-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.