http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110951672-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-395 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2513-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-392 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0625 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5044 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
filingDate | 2019-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110951672-B |
titleOfInvention | Mouse endometrial epithelial cell and construction method of 3D differentiation culture model thereof |
abstract | The invention discloses a mouse endometrial epithelial cell and a construction method of a 3D differentiation culture model thereof. The mouse normal endometrium epithelial cell is named as MNUEC/HL-027, and the preservation number is CCTCC No: C2015108. meanwhile, a construction method of the mouse endometrial epithelial cell 3D differentiation culture model is provided: and (3) suspending single cells by the aid of a 2D culture medium, inoculating the single cells into a gas-liquid culture device, and culturing for 14-21 days by replacing the 2D culture medium with a 3D differentiation culture medium. After the 3D differentiation culture of the mouse endometrium epithelium is completely differentiated, the mouse endometrium epithelium has similar structural characteristics with normal endometrium tissue, and a pseudo-multilayer structure consisting of epithelial cells is formed, and comprises a basal layer and a functional layer. Meanwhile, the recombinant human endometrial peptide also has the expression characteristics of specific protein markers and cell tight junction related proteins which are the same as those of normal mouse endometrial tissues, has structural functionality, and can be used for physiological research and functional research of regeneration and repair of the endometrial tissues. |
priorityDate | 2019-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 260.