| abstract |
The present invention relates to a recombinant human interferon α2a suppository and a preparation method thereof, wherein the recombinant human interferon α2a is a recombinant plasmid containing human interferon α2a gene constructed by using Escherichia coli expression technology, and transformed into Escherichia coli to obtain stable engineering bacteria , recombinant human interferon α2a is obtained by purification after engineering bacteria fermentation, and mixed with glycerol and gelatin matrix to make recombinant human interferon α2a suppository after sterile filtration; It is an excipient and a bacteriostatic agent; gelatin is not only an excipient, but also a protease inhibitor, which can effectively protect the protein of recombinant human interferon α2a from being degraded, ensure its structural integrity, and stabilize the biological activity of interferon. The protease that can interfere with the release of cells further damages the damaged mucosa of the vagina, and has the effect of protecting the mucosa. |