http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110938690-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fd2fe67d327d5f05f41069243ef940c4 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6825 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6825 |
| filingDate | 2019-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ecefec8270246eacda6008aa3c617e7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc8ac996979b5973cb084f7acc896ad0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee5773b2a93804fa0b370596c99101e0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b2921060887445e108badb28a0e32289 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9bb3512e2787a9055212c02fc91c24b8 |
| publicationDate | 2020-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110938690-A |
| titleOfInvention | Label-free colorimetric sensor based on target-triggered enzyme-assisted hairpin probe remodeling and its applications |
| abstract | Label-free colorimetric sensor based on target-triggered enzyme-assisted hairpin probe remodeling and its application, targeting nucleic acids, reacting with the aid of enzymes and forming G-quadruplexes that can generate colorimetric signals in multiple ways Sequence, a method for visual detection of nucleic acids. In this method, the reaction conditions are controlled, and the hairpin probe (HGP) opened by the target gene under the action of T4 DNA ligase is connected with another double-stranded DNA (DGP) to complete the remodeling. Then, under the synergistic action of DNA polymerase and restriction endonuclease, the target DNA is recycled and multiple channels for generating G-quadruplex sequences are generated. The present invention uses enzyme-assisted DNA reaction to form a large number of G-quadruplexes, which can be detected by an ultraviolet spectrophotometer, and can also be visually detected by naked eyes. The detection method is convenient, the materials used have been commercialized, and the biological safety is high. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111879926-A |
| priorityDate | 2019-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 103.