http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110938640-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y504-9904 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-61 |
filingDate | 2019-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110938640-B |
titleOfInvention | Alpha-amyrin synthetase gene EjAAS1 and application thereof |
abstract | The invention discloses an alpha-amyrin synthetase gene EjAAS1 and application thereof, and particularly discloses application of a coding gene with a nucleotide sequence shown as SEQ ID NO.1 as loquat alpha-amyrin synthetase gene and application of a protein with an amino acid sequence shown as SEQ ID NO.2 as loquat alpha-amyrin synthetase. The invention firstly clarifies the alpha-amyrin synthetase gene EjAAS1 with the expression change in different tissues and the content of triterpenic acid being obviously and positively correlated, the gene can be expressed instantly in tobacco leaves to heterologously synthesize alpha-amyrin in the tobacco leaves, and the gene can promote the synthesis of loquat alpha-amyrin and the in vitro biosynthesis of alpha-amyrin and derivatives of ursolic glycoside triterpenic acid. The invention lays a foundation for the application of synthetic biology methods such as gene tandem expression and a biological generator in the research of loquat metabolism, and provides theoretical support for the modification and utilization of functional components. |
priorityDate | 2019-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 469.