http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110935036-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_72de4ac6b66c3ef9bf8ed356540a7702 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K49-0004 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K49-00 |
| filingDate | 2019-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4e0bf25e61733e02fa8977ca71c9295c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c6a4c1d349b66227e2a8739de2d81548 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a321297d2c268cdc962269567d70ffb6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c5e72c86874da50d82d9e1b3579cc928 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_339859367a2e5897a5ce026e3598ec2f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77f2c9eab716b2a9d66b02dbf095efc3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a58f1078dc102261bf7cb944247b268e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7033947113b3774cf9915ebeaba16a67 |
| publicationDate | 2020-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110935036-A |
| titleOfInvention | A method for evaluating the effects of RNAi transgenic products on Italian honeybees |
| abstract | The invention discloses a method for evaluating the influence of RNAi transgenic products on Bee mellifera. First, dsRNA is synthesized, which includes the following steps: Step 1: Primer design: using SMV-P3-RNAi gene transgenic soybean B5B9013 as test material, according to The SMV-P3 gene forward interfering fragment sequence and the GFP gene sequence in B5B9013 soybean, primers were designed, and a DNA template for dsRNA synthesis was obtained by amplification; Step 2: dsRNA reverse transcription: use MEGAscript transcription kit to synthesize dsRNA. The present invention uses SMV-P3-RNAi gene transgenic soybean B5B9013 as test material, designs primers according to the SMV-P3 gene forward interference fragment sequence and GFP gene sequence in B5B9013 soybean, and amplifies to obtain a DNA template for dsRNA synthesis , The MEGAscript transcription kit was used to synthesize dsRNA, and the effect of dsRNA on bees was tested. The test results showed that the dsRNA expressed could improve the survival rate of the economic insect silkworm during the safety evaluation of RNAi transgenic crops. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110295198-A |
| priorityDate | 2019-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.