http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110927393-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9b27e9c77a9264100b9a5cecbb1e8882 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1a191085cb526c4aadf3c340b159fc7c |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-042 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-805 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-721 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2019-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4319d88bbebe424597f8cc065f2861f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c962cd6e38b576e6051332fdedd1164 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5922c5204c309b8fb3707fcace6ea5d3 |
| publicationDate | 2020-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110927393-A |
| titleOfInvention | A method for quantifying non-enzymatic modification sites of hemoglobin by LC-PRM/MS and its application |
| abstract | The invention discloses a method for quantifying non-enzymatic modification sites of hemoglobin by using LC-PRM/MS and its application. The method includes: 1) incubating a small molecule standard solution with hemoglobin in vitro to obtain a modified hemoglobin sample in vitro; 2) extracting hemoglobin in human blood; 3) using LC-MS/MS to separate trypsin, chymotrypsin and hemoglobin, respectively. The in vitro and in vivo hemoglobin peptides obtained after the enzymatic hydrolysis of Staphylococcus aureus V8 protease were detected; 4) the information distribution of hemoglobin modification sites was obtained from the detection results and the modification site spectral library was established; 5) the hemoglobin after enzymatic hydrolysis was Peptide fragments were mixed for monitoring experiments; 6) According to the modified site spectrum library, LC-PRM/MS was used to screen peptide markers for early diagnosis of diabetes. The invention can improve the coverage of hemoglobin modification sites, obtain accurate quantitative results, and is expected to provide a reference method for early screening of diabetes. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112051342-A |
| priorityDate | 2019-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 100.