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filingDate 2019-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f6c4963f2197e1ce2ec046f5aebcef2e
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publicationDate 2020-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110927375-A
titleOfInvention A kind of fluorescent microsphere immunochromatographic test strip for detection of olaquindox residue and its application
abstract The invention discloses a fluorescent microsphere immunochromatographic test strip for detecting olaquindox residue and its application. The test strip of the invention can qualitatively and quantitatively detect olaquindox in water and feed, and the sample pretreatment process is simple. , convenient and quick, the detection time is short, and the test strip has high precision and sensitivity; and the anti-Oquindox monoclonal antibody prepared by the invention has strong specificity, and the anti-Oquindox monoclonal antibody labeled with fluorescent microspheres is used. The luminescence intensity and detection signal of the sphere can be enhanced with the increase of the excitation light intensity, so the fluorescent microsphere-labeled anti-Oquindox monoclonal antibody can effectively improve the analytical sensitivity of immunochromatography, compared with traditional colloidal gold immunochromatography , the immunoassay method of the present invention has higher sensitivity; at the same time, due to the relatively stable morphological structure of the fluorescent microspheres, the uniform particle size of the microspheres, the good dispersion and stability, the high luminous efficiency, the good repeatability, and the large dye fluorescence quenching reduce.
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