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publicationDate 2020-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110927312-A
titleOfInvention A kind of construction method of UPLC characteristic map of Meihua formula granule and its component content determination method
abstract The invention relates to a method for constructing a UPLC characteristic map of Meihua formula granules and a method for measuring the content of components. The method for measuring the content of components of Meihua formula granules includes the following steps: (1) respectively drawing a reference solution and a plum blossom formula to be tested The particle sample solution was injected into an ultra-high performance liquid chromatograph to determine the corresponding peak area; (2) the content of hyperoside was calculated by the external standard method; (3) chlorogenic acid and isoquercitrin were calculated by the one-test-multiple-evaluation method. , the content of rutin. By optimizing the chromatographic conditions and the preparation of the test sample, the present invention calculates the content of hyperin by using the external standard method, and then calculates the content of chlorogenic acid, isoquercitrin and rutin by one measurement and multiple evaluation method, and can The operation steps of the content determination of the four components are effectively simplified, the determination time is reduced, the work efficiency is improved, and the qualitative and quantitative analysis of the chemical components of the Meihua formula granules is realized.
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