| abstract |
The invention discloses a kit for detecting short-chain fatty acids, comprising a box body, an enzyme label plate, a short-chain fatty acid standard gradient solution, a sample diluent, and an alkaline phosphatase-labeled detection anti-short-chain fatty acid set in the box body. The antibody of the conjugate, 20× concentrated washing solution, chromogenic reagent, stop solution and sealing film, sealing bag and instructions; wherein, the ELISA plate is a transparent polystyrene 96 or 48-well ELISA plate, and each of its The wells are coated with the anti-short-chain fatty acid monoclonal antibody Mab1 at a concentration of 5 μg/mL; the short-chain fatty acid standard gradient solution has 6 concentrations of solutions, 20ng/mL, 10ng/mL, 5ng/mL, 2.5 ng/mL, 1.25ng/mL, 0ng/mL. The detection kit of the present invention provides an enzyme-labeled secondary antibody labeled with alkaline phosphatase (ALP), which significantly improves the stability and sensitivity of the kit. |