| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_07a5fd9fffa3caec61fd03b8b7bf6f8e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_164f142bbfd407b59d4a635d2468829f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f535660b0a0e565cf1ec41411bae18e5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2e770006a93254a4c7894446b7175f23 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-118 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886 |
| filingDate |
2018-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d740c8b182f6d3d9635f2d601e82beca
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_25ffd19f94f75089aac812d5504e83eb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ef1f69130a15e92583d43a800286bf5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b18cc12d94d8ea50dff2ec4c97aa45d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bbe070d2e1699048eeaefd4c9454008a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1498babf8251e0c4096b5825c0daf7e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a8948a98ec28c312051b798d5592c55 |
| publicationDate |
2020-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-110904512-A |
| titleOfInvention |
A high-throughput sequencing library construction method suitable for single-stranded DNA |
| abstract |
The invention discloses a high-throughput sequencing library construction method suitable for single-stranded DNA. The method of the present invention comprises: connecting a single-stranded template with a double-stranded linker 1 with a degenerate base (a special linker with a degenerate base makes it possible to connect the single-stranded template with the double-stranded linker, and the double-stranded linker can be randomly Bind to the single-stranded template, make the linker sequence sufficiently close to the template, and then use T4 DNA ligase to complete the connection); use the extension primer to extend to obtain a double-stranded product; connect the double-stranded product with the linker 2; The obtained ligation product is Template, PCR amplification was performed to obtain the desired library. The present invention can be used to construct high-throughput sequencing libraries, and can also be used to construct single-stranded DNA libraries. The present invention will improve the utilization rate of single-stranded DNA in the construction of high-throughput sequencing libraries, and further realize the purpose of building libraries with low starting amount. In the future It plays a key role in early tumor screening and prognostic monitoring applications. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112410331-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113481196-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113249437-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113529177-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111455469-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111455469-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112941073-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112941073-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116103383-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111979583-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111979583-B |
| priorityDate |
2018-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |