http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110904075-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2402 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01037 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 |
| filingDate | 2019-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110904075-B |
| titleOfInvention | Salt-tolerant xylosidase mutant K321D and preparation method and application thereof |
| abstract | The invention discloses a salt tolerant xylosidase mutant K321D, a preparation method and application thereof, wherein the amino acid sequence of the mutant K321D is obtained by mutating 321 th lysine of wild xylosidase HJ14GH43 into aspartic acid, the sequence is shown as SEQ ID NO.1, and the salt is not NaCl. Compared with the wild enzyme HJ14GH43, the mutant enzyme K321D of the invention has high KCl and Na concentration 2 SO 4 And (NH) 4 ) 2 SO 4 The stability of the composition is enhanced, the activity of the composition is increased from 48% to 65% by KCl treatment with the concentration of 20.0-30.0%, and the composition is subjected to Na treatment with the concentration of 10.0-30.0% 2 SO 4 Treating, wherein the activity is 93-111%; (NH) at a concentration of 20.0-30.0% 4 ) 2 SO 4 The activity of the treatment is maintained above 90%, and the treatment method can be applied to industries such as leather making, papermaking and sewage treatment. |
| priorityDate | 2019-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 361.