| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7d0451e593692e9996ee1672449fb2fe |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2513-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-90 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0693
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-507 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate |
2019-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c6fbb24336dbd145f03133133fc09ba
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e645ac163d77e767abc4b3356ad23f56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_602e2ee87c65184c12ebcf2609a35ca5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19b1719e9d70229c54963f3b37635dea
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ebbc3ec626ead235315058848a733e42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aaf9ea014c6c940712211a0362bd836d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90ed89476a5a85875094017a522cded8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a98d5cbd0ab33ed410e4993adbb685d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3758d450a12b0405be1791ff56ed564b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f11929537ac0387d9cc8af4cf3f7aa90 |
| publicationDate |
2020-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-110894492-A |
| titleOfInvention |
A method for constructing an in vitro 3D model of pancreatic cancer based on pancreatic decellularized scaffolds |
| abstract |
A method for constructing an in vitro 3D model of pancreatic cancer based on a pancreatic decellularized scaffold, belonging to the technical field of tissue engineering and tumor invasion, metastasis and drug resistance; a pancreatic acellular scaffold based on a simulated tumor microenvironment in vivo, after replanting pancreatic cancer cells to construct a 3D culture model of pancreatic cancer in vitro. On the basis of traditional 2D culture based on cell-cell interaction, a cell-extracellular matrix and a three-dimensional space structure are introduced, in which tumor cells can grow in a three-dimensional structure, showing round and oval cell shapes, and Changes in nucleocytoplasmic ratio and mitotic figures can be seen. The construction of this model includes the following steps: (1) acquisition of pancreatic tissue; (2) treatment by hydration decellularization method and evaluation of the decellularization effect; (3) cobalt-60 irradiation sterilization; (4) pancreatic cancer cell replantation and functional evaluation. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114317425-A |
| priorityDate |
2019-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |